P4-029: CHARACTERIZING AFFINITY EPITOPES BETWEEN FIBRINOGEN AND β-AMYLOID USING AN EPITOPE MAPPING IMMUNOASSAY
نویسندگان
چکیده
منابع مشابه
Epitope Mapping: B-cell Epitopes
Immunoglobulin molecules are folded in a way that assembles sequences from the variable regions of both the heavy and light chains into a surface feature (comprised of up to six complementarity-determining regions (CDRs)) that is complementary in shape to a surface structure on the antigen. These two surface features, the ‘paratope’ on the antibody and the ‘epitope’ on the antigen, may have a c...
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UNLABELLED Identifying the epitope to which an antibody binds is central for many immunological applications such as drug design and vaccine development. The Pepitope server is a web-based tool that aims at predicting discontinuous epitopes based on a set of peptides that were affinity-selected against a monoclonal antibody of interest. The server implements three different algorithms for epito...
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The majority of patients with Alzheimer disease (AD) suffer from impaired cerebral circulation. Accumulating evidence suggests that fibrinogen, the main protein component of blood clots, plays an important role in this circulatory dysfunction in AD. Fibrinogen interacts with β-amyloid (Aβ), forming plasmin-resistant abnormal blood clots, and increased fibrin deposition is found in the brains of...
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As antibody-based diagnosis and therapy grow at an increased pace, there is a need for methods which rapidly and accurately determine antibody-antigen interactions. Here, we report a method for the multiplex determination of antibody epitopes using bacterial cell-surface display. A protein-fragment library with 10(7) cell clones, covering 60 clinically-relevant protein targets, was created and ...
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Introduction CINCA syndrome, also known as NOMID, is a rare autoinflammatory disease caused by the NLRP3 mutations. It has been known that conventional genetic analysis failed to detect disease-causing mutations in approximately 40% of patients. We have recently identified NLRP3 somatic mosaicism on 70% of these ”mutation-negative” patients in the international collaborative study (Tanaka N. an...
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ژورنال
عنوان ژورنال: Alzheimer's & Dementia
سال: 2019
ISSN: 1552-5260
DOI: 10.1016/j.jalz.2019.06.3688